Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.133318 |
Chromosome: | chromosome 4 |
Location: | 3869163 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g230340 | FAP206 | (1 of 1) PTHR21442:SF0 - UPF0704 PROTEIN C6ORF165; Flagellar Associated Protein 206 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTCTTCCCTTCCCCGTCCTCTACACAC |
Internal bar code: | GGGCGAGGGGCATACCACCCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 90 |
LEAP-Seq percent confirming: | 99.4118 |
LEAP-Seq n confirming: | 676 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTACCAGCAACCCACACTT |
Suggested primer 2: | CCGCCAGTGTAACTTGGTTT |