| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.133334 |
| Chromosome: | chromosome 15 |
| Location: | 1267121 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641650 | HLM22 | (1 of 4) PTHR10887:SF331 - HELICASE MOV10L1-RELATED; Histone-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACATACAACTCAAGCAAAACGCAACAG |
| Internal bar code: | TTTCAGGGACCCAAGTTGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 54.71 |
| LEAP-Seq n confirming: | 1330 |
| LEAP-Seq n nonconfirming: | 1101 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTACTGCCTGTGAGGGG |
| Suggested primer 2: | GAGGTGTGGAAGGACTGGAG |