Insertion junction: LMJ.RY0402.133341_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.133341
Chromosome:chromosome 16
Location:4478314
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g670756 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGTGCCCATGACTTCCCAACCTGTGATG
Internal bar code:ACTGGTCGCCATGGCTGTAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:830
LEAP-Seq percent confirming:99.7317
LEAP-Seq n confirming:1487
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTTCAACTGCTCTTTCCC
Suggested primer 2:AGGCGCAGTATAGGAAAGCA