| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133353 |
| Chromosome: | chromosome 12 |
| Location: | 2579181 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505300 | (1 of 1) PF08317 - Spc7 kinetochore protein (Spc7) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGGCATTCACGTAGCTTTGTTGTAATCA |
| Internal bar code: | CAGCGGCGCACCTAACATTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 89.1892 |
| LEAP-Seq n confirming: | 396 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATAGGCGTTTACTGGGC |
| Suggested primer 2: | GACGTTCATGGTGATGTTGC |