| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133354 |
| Chromosome: | chromosome 1 |
| Location: | 3970858 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025808 | OPR122 | OctotricoPeptide Repeat protein 122 | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAAACCAAGGCCAACGAAAACGGGTGTG |
| Internal bar code: | GGAGATCTGAGGCGTCGTTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1088 |
| LEAP-Seq percent confirming: | 74.8244 |
| LEAP-Seq n confirming: | 4580 |
| LEAP-Seq n nonconfirming: | 1541 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGTGGTCATTTGATGTG |
| Suggested primer 2: | GCTGGTAATGTGCTAGGCAG |