| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133358 |
| Chromosome: | chromosome 9 |
| Location: | 3300456 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388208 | (1 of 1) 1.13.11.24 - Quercetin 2,3-dioxygenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGGTAGGCCTGTCGCCGCGGCCCCAGG |
| Internal bar code: | GCACTCCTGGCTCCGGTGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 272 |
| LEAP-Seq percent confirming: | 99.6059 |
| LEAP-Seq n confirming: | 5308 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCTGGGGAATAGCCGTC |
| Suggested primer 2: | TATGAGCAACAGTCCGCTTG |