| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133369 |
| Chromosome: | chromosome_7 |
| Location: | 5573958 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g351650 | FAP20,BUG22 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGACCTGAGAGAGCGAGCCATGTTCAAGA |
| Internal bar code: | CGGTCGGCGTGAAGGTAAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 473 |
| LEAP-Seq percent confirming: | 98.5705 |
| LEAP-Seq n confirming: | 4620 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAATAGCCGGCAAGCAAAG |
| Suggested primer 2: | CTCGATGTAGTTCGTCCCGT |