| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133369 |
| Chromosome: | chromosome_16 |
| Location: | 4461871 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| None |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCGCCCGGTGTACTTTGCCATCAGGGGTC |
| Internal bar code: | CCGATTATCGCTCCTCTCTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 99.4991 |
| LEAP-Seq n confirming: | 3774 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGAGACATTACTGACCGT |
| Suggested primer 2: | CACCATGTGAAGCGGTATTG |