| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133373 |
| Chromosome: | chromosome 8 |
| Location: | 3386833 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374350 | (1 of 6) PF01847 - von Hippel-Lindau disease tumour suppressor protein (VHL) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTTCGATCCTGGATGGTGACAGTTTTGC |
| Internal bar code: | CCGGCACGGCGTGATGGGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 944 |
| LEAP-Seq percent confirming: | 99.895 |
| LEAP-Seq n confirming: | 3807 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACTCTATTCCACCGGCA |
| Suggested primer 2: | TAAACTACGGTGGCAGTCCC |