Insertion junction: LMJ.RY0402.133375_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.133375
Chromosome:chromosome 9
Location:2936432
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g387950 (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAACGTTCCCTGCACCCGCGCCGCTCAC
Internal bar code:CCCCGCTGTGGCCGTCGAACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:148
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGATGTTCTTCACATGACGG
Suggested primer 2:CACCAGCGTGAACAAGAGAA