| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133377 |
| Chromosome: | chromosome 6 |
| Location: | 5719094 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286850 | (1 of 1) 1.1.1.193 - 5-amino-6-(5-phosphoribosylamino)uracil reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGAGAGCGGTGTGAGGGATGTGTGGA |
| Internal bar code: | ACATACGGCATCCGTCATCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 822 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 182 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCACAACTACAGCCAAA |
| Suggested primer 2: | GCGGTGATGGAGGAGATTTA |