Insertion junction: LMJ.RY0402.133397_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.133397
Chromosome:chromosome 2
Location:3238502
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095076 ROC39,MFT10 Major facilitator superfamily transporter; (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAACCCGTGCCCGCGCTCGTGCCCGAAC
Internal bar code:ACCAGGAAGTTGACACGCTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:119
LEAP-Seq percent confirming:60.5263
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTTAAGAACGAAACGCTCGC
Suggested primer 2:TTCCTCCACAATTGAAAGCC