Insertion junction: LMJ.RY0402.133412_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.133412
Chromosome:chromosome 6
Location:6957142
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g296300 SRR1 Scavenger receptor cysteine rich (SRCR) protein; (1 of 20) IPR001190//IPR017448 - SRCR domain // SRCR-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGACCCCGCCGCACACGCCTTCTGGGCGC
Internal bar code:GTCCGCCCACAGATTGTCGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:398
LEAP-Seq percent confirming:90.3017
LEAP-Seq n confirming:419
LEAP-Seq n nonconfirming:45
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AGTACATAAGCCGCACCACC
Suggested primer 2:CCACACCTGAAGGTCCAACT