| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133414 |
| Chromosome: | chromosome 16 |
| Location: | 1402535 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652200 | MMP9 | Metalloproteinase of VMP family; (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCAGCCCTGGGGAGGGTGTGTGCAGCA |
| Internal bar code: | CGAGTGCTGCCCGATCCCCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1078 |
| LEAP-Seq percent confirming: | 99.5333 |
| LEAP-Seq n confirming: | 7038 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTGCTTGTCATTTTGGT |
| Suggested primer 2: | TTGCAAGACTTACAGGCACG |