| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133444 |
| Chromosome: | chromosome 6 |
| Location: | 2166317 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265950 | DHC3 | Axonemal Inner Arm Dynein Heavy Chain 3, pseudogene; (1 of 2) PTHR10676:SF136 - DYNEIN HEAVY CHAIN 6, AXONEMAL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGCGGCAGCTGCCAGCAGCTGCTCCGC |
| Internal bar code: | GCGGTCGCGGTGGGTAAAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 448 |
| LEAP-Seq percent confirming: | 98.0482 |
| LEAP-Seq n confirming: | 4923 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCTCCACCAGGAACATCT |
| Suggested primer 2: | GAGCTGTTCACCGACTCCTC |