Insertion junction: LMJ.RY0402.133447_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.133447
Chromosome:chromosome 7
Location:3609884
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g336750 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCCTTGTGTGCGTGTGCGCCACCCATT
Internal bar code:GCGTGTCAATATCAAGTTTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:81
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGATACACACAAAGCGCACA
Suggested primer 2:AGACAGGACTGAGCACCCAC