Insertion junction: LMJ.RY0402.133483_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.133483
Chromosome:chromosome 5
Location:761963
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g248550 (1 of 1) K16276 - zinc finger protein-like protein (K16276, BTS) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGGAGGGGGAGAGCACAGTGTTGATCCG
Internal bar code:GGGAATCTAGTAGGGGCCGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:145
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGTAACAAGCACGCACAGTT
Suggested primer 2:AGCACCTGCTTCTCCACCTA