Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.133484 |
Chromosome: | chromosome_8 |
Location: | 4265881 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g380950 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TAGGAACATCAAGCAAGAGCACAAACACAG |
Internal bar code: | GGTGGGGCGCGGATCATTCCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 846 |
LEAP-Seq percent confirming: | 99.7325 |
LEAP-Seq n confirming: | 1864 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACATTTCAGTTTGTGCAGCG |
Suggested primer 2: | ACCAATGTGGTGGTGGATGT |