| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133489 |
| Chromosome: | chromosome_16 |
| Location: | 5363536 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g681750 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGACCCGCCTTCTAGGCTTTATTGTGCTG |
| Internal bar code: | TGGGTCGGGATTTGTACAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1097 |
| LEAP-Seq percent confirming: | 98.9761 |
| LEAP-Seq n confirming: | 4060 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACACACACACCTCCA |
| Suggested primer 2: | GTCTTGCTGAGGTTCCTTGC |