Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.133493 |
Chromosome: | chromosome_12 |
Location: | 748398 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g495350 | POC12 | Centriole proteome protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGGGTGGTGGCGACAGTGGGGTTAGGCCG |
Internal bar code: | GTGTAATGCCGGTCCCGTTCTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 359 |
LEAP-Seq percent confirming: | 98.9257 |
LEAP-Seq n confirming: | 4604 |
LEAP-Seq n nonconfirming: | 50 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGTCCCTTGCTCAGCCAGTA |
Suggested primer 2: | GATGCATGAGCGGTGAGTAA |