Insertion junction: LMJ.RY0402.133496_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.133496
Chromosome:chromosome 1
Location:1496804
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g007700 (1 of 1) 3.4.11.1//3.4.11.5 - Leucyl aminopeptidase / Peptidase S // Prolyl aminopeptidase / Proline iminopeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCATACACACACACGCGAGCACATGGA
Internal bar code:TAATTCACAGTTACCCGGTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:614
LEAP-Seq percent confirming:99.4459
LEAP-Seq n confirming:6820
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCGATTCTCTGCATGTAT
Suggested primer 2:TCACCAATCCGAATACGACA