| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133504 |
| Chromosome: | chromosome 10 |
| Location: | 1858365 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431350 | TGL16 | (1 of 4) PTHR12406//PTHR12406:SF27 - CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATED // SUBFAMILY NOT NAMED; Putative triacylglycerol lipase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTGCCACTGGACACAACGAATGAGGCTG |
| Internal bar code: | CGCGGAAGCTTTTTGTGAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 961 |
| LEAP-Seq percent confirming: | 93.0832 |
| LEAP-Seq n confirming: | 7765 |
| LEAP-Seq n nonconfirming: | 577 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACAGGGCGTAGGTGTGT |
| Suggested primer 2: | CATGCAAAGGTGCCACATAC |