| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133518 |
| Chromosome: | chromosome 14 |
| Location: | 2196906 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g622700 | UMAMIT (Usually Multiple Acids Move In and Out Transporters) type transporter; (1 of 5) PTHR11132//PTHR11132:SF101 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCAGGATGAAATTCCAGTGGGTCTGGG |
| Internal bar code: | CGACTTATTTCCTGGCAGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 246 |
| LEAP-Seq percent confirming: | 99.674 |
| LEAP-Seq n confirming: | 5504 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCAAGGAACTGTCGCTG |
| Suggested primer 2: | TGTGGCACAAAGCGTCTATC |