| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133523 |
| Chromosome: | chromosome 7 |
| Location: | 5046366 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347400 | (1 of 2) K11135 - Pin2-interacting protein X1 (PINX1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTGTGGAGCGTGAGGCAGAGGATGTGT |
| Internal bar code: | GATTTGCGGGAGCAACTGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 969 |
| LEAP-Seq percent confirming: | 99.8458 |
| LEAP-Seq n confirming: | 30442 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 119 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCTCATCCACACGTTCA |
| Suggested primer 2: | CTGTTACTGGAGCACCCCAT |