Insertion junction: LMJ.RY0402.133533_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.133533
Chromosome:chromosome 6
Location:419890
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g252200 TOC34 Translocon at the outer envelope membrane of chloroplasts, receptor subunit; (1 of 1) PTHR10903//PTHR10903:SF43 - GTPASE, IMAP FAMILY MEMBER-RELATED // GTP-BINDING PROTEIN A 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTGTCCCCTTTGCCGGCCCCATCGTACA
Internal bar code:ATCCGATCATCAGTGGGTCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:443
LEAP-Seq percent confirming:99.4212
LEAP-Seq n confirming:1546
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGCATATGATCGGAGGG
Suggested primer 2:TGGAGAACAGTGAGACGTGC