| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133557 |
| Chromosome: | chromosome 12 |
| Location: | 9314504 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540850 | (1 of 1) IPR009031 - Carbohydrate binding module family 10 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGAGCCGTTCAGGCATTCCGGCACCCA |
| Internal bar code: | TTCCTTCATTAAGGCACTTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 99.4681 |
| LEAP-Seq n confirming: | 1122 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGGAAGTCGTAGAGCGG |
| Suggested primer 2: | ACGCAACACATCAGCAGAAC |