| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133566 |
| Chromosome: | chromosome 1 |
| Location: | 4615321 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031750 | (1 of 1) K11883 - RNA-binding protein NOB1 (NOB1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAACTCATGTCATCCTTCGCCGCTGCGC |
| Internal bar code: | GGGCAACACGACCCATCGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 172 |
| LEAP-Seq percent confirming: | 0.744806 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 2532 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGTGGGAGGGCTAAGTT |
| Suggested primer 2: | CCGGCATAGCATGTAGGAAT |