| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133580 |
| Chromosome: | chromosome 2 |
| Location: | 2851127 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGCAACCTGCACACACGCCTGGTGTT |
| Internal bar code: | GCCAAATGTCTCCGGTCGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 568 |
| LEAP-Seq percent confirming: | 83.5311 |
| LEAP-Seq n confirming: | 5138 |
| LEAP-Seq n nonconfirming: | 1013 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAAACTTGGCACGAACC |
| Suggested primer 2: | CAAGCAAGGTTACGACAGCA |