| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133581 |
| Chromosome: | chromosome 11 |
| Location: | 217547 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467552 | (1 of 3) K00850 - 6-phosphofructokinase 1 (pfkA, PFK) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCAATCCCGTAAGGAAACAGTGGAGGAG |
| Internal bar code: | GTCGAACTCCTTTGACAAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 98.6426 |
| LEAP-Seq n confirming: | 4651 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTAACACGGCACCACTC |
| Suggested primer 2: | CAAGGTATACAGCCCCTCCA |