| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.133586 |
| Chromosome: | chromosome 5 |
| Location: | 2651689 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236500 | (1 of 1) 3.5.1.23 - Ceramidase / Acylsphingosine deacylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCGACGTCAGGGGAGAGGCCAGCGGGA |
| Internal bar code: | ACGGTCACACGGGGTCGCCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 99.8031 |
| LEAP-Seq n confirming: | 4563 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTGGGCCTGTACACAT |
| Suggested primer 2: | GTAGCCTGTGCCCAATGTTT |