Insertion junction: LMJ.RY0402.133587_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.133587
Chromosome:chromosome 1
Location:2858560
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g017200 FKB24,FKB99 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 2) PTHR10516//PTHR10516:SF290 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // PEPTIDYL-PROLYL CIS-TRANS ISOMERASE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGGGGTGTTAGTTGCTCATGTGGAGAAC
Internal bar code:GCGCGGCGGATTTATTCTGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:298
LEAP-Seq percent confirming:99.0547
LEAP-Seq n confirming:2934
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGATATGAGCTCCACCTCCA
Suggested primer 2:TGCTACGTACACGAGCAAGC