| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133591 |
| Chromosome: | chromosome_16 |
| Location: | 4976605 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g685250 | SUB11 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGAGAGATCGTGTGACCTGCAAGAGTTGAG |
| Internal bar code: | GCCTAGATTGGGGGGCGGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 98.5805 |
| LEAP-Seq n confirming: | 4792 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACCACCCAGCCACTACA |
| Suggested primer 2: | ACAACATTTCCTTTGCCAGC |