| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133594 |
| Chromosome: | chromosome 12 |
| Location: | 2300593 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507700 | (1 of 1) K12879 - THO complex subunit 2 (THOC2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCTGTAAGGCGCACGTGCATAAGCAAT |
| Internal bar code: | AGCCGTGTCGTCGGGGTGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1274 |
| LEAP-Seq percent confirming: | 99.5884 |
| LEAP-Seq n confirming: | 8468 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTTAGGGCGCTGTCTTC |
| Suggested primer 2: | GGCTTTTATAGCCCCGTTTC |