| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133600 |
| Chromosome: | chromosome 1 |
| Location: | 7992030 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g069472 | TSC2 | Putative organellar cysteinyl tRNA synthetase; (1 of 2) 6.1.1.16 - Cysteine--tRNA ligase / Cysteinyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATCTGAAGTTGCATACAGGCATTCCAGC |
| Internal bar code: | CGGTAAGGGTTTCCTGCCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 99.6061 |
| LEAP-Seq n confirming: | 6575 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | TCCCACGGTATCGACTTTTC |