| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133603 |
| Chromosome: | chromosome 3 |
| Location: | 6623766 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196700 | (1 of 4) PF01679 - Proteolipid membrane potential modulator (Pmp3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCTGCGCTGTTGATGCGTGACAACCTG |
| Internal bar code: | GAGGGCAACGTAAAATCGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 99.4946 |
| LEAP-Seq n confirming: | 4725 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCTGCTGCTGTCCATGT |
| Suggested primer 2: | GCACAGAAGTGCATTGGCTA |