| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.133613 |
| Chromosome: | chromosome 3 |
| Location: | 7836915 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202850 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGCGGCGATGCTTCTGGGCAGCACTT |
| Internal bar code: | TCTCGGAGGGGCTAACGGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 30 |
| LEAP-Seq percent confirming: | 1.62304 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 3758 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCAGTTGTCACCTTGAGT |
| Suggested primer 2: | GTCAGTCAGTCGCAAGTCCA |