| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.133614 |
| Chromosome: | chromosome_1 |
| Location: | 4325320 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g029250 | AAH1 | Aromatic amino acid hydroxylase-related protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGAATGTCTTCGGGGATGGGAGGCCTCGT |
| Internal bar code: | GATCATCCTAATCGCGCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 655 |
| LEAP-Seq percent confirming: | 99.275 |
| LEAP-Seq n confirming: | 8490 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGAAGAACGTTCGGAGC |
| Suggested primer 2: | GCAACATCAGGAGTCAGCAA |