| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133626 |
| Chromosome: | chromosome 12 |
| Location: | 5213303 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527950 | (1 of 1) 2.4.1.227 - Undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase / Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase; Undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGAGAGGTGGGCAGGGGTGCATGGTGT |
| Internal bar code: | AATGAGGACGAGAGCACTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 463 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 373 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCGCATTACGGTATGCAC |
| Suggested primer 2: | AATGCTTGGTATCCCTCGTG |