| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133629 |
| Chromosome: | chromosome 12 |
| Location: | 5734580 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532950 | (1 of 4) PF00060//PF00497 - Ligand-gated ion channel (Lig_chan) // Bacterial extracellular solute-binding proteins, family 3 (SBP_bac_3); Ligand-gated ion channel | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGGTATCTTTATCCCACCGATACCGGT |
| Internal bar code: | CGTGGGGTACCAACCGATAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 159 |
| LEAP-Seq percent confirming: | 79.5366 |
| LEAP-Seq n confirming: | 14074 |
| LEAP-Seq n nonconfirming: | 3621 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATCAAGCAAACAGCAGGA |
| Suggested primer 2: | GGCACAAAATGGGTACTGCT |