Insertion junction: LMJ.RY0402.133651_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.133651
Chromosome:chromosome 1
Location:3643345
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g023550 FAP234,TPG2 Flagellar Associated Protein 234; (1 of 1) PTHR24365//PTHR24365:SF301 - TOLL-LIKE RECEPTOR // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTGCAAGGCTGTGCGCGGCTGCCTGTGA
Internal bar code:TTTCCGCTTCACTTTTACTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:399
LEAP-Seq percent confirming:99.8274
LEAP-Seq n confirming:11567
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:AAATGATGAGGTTTGCGAGG
Suggested primer 2:CTCAGCTTCTGCGGTAGCAC