| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133654 |
| Chromosome: | chromosome 10 |
| Location: | 6251074 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464800 | (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGGGTTGTAACAGGATGGAGCTCTCAT |
| Internal bar code: | AATTCCAGATGCGTGTGGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 99.7925 |
| LEAP-Seq n confirming: | 5770 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGTGGACTTAGTATCGGA |
| Suggested primer 2: | GTTGGCTTGTCGGTCAGAAT |