| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133666 |
| Chromosome: | chromosome 12 |
| Location: | 9077060 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542900 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCACGTGCTCCAGCTGCAGCTGCGTCAC |
| Internal bar code: | AGCGTGTTGTCATCGATCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 68.9502 |
| LEAP-Seq n confirming: | 6049 |
| LEAP-Seq n nonconfirming: | 2724 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACATTGGAGTGAAGCGGT |
| Suggested primer 2: | CAGAGGCTTTCTCATCTGGG |