| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133688 |
| Chromosome: | chromosome 3 |
| Location: | 5674789 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187600 | (1 of 15) PF13365 - Trypsin-like peptidase domain (Trypsin_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTACACCACCTCCGCCTCCACGTGCTGC |
| Internal bar code: | GATCACAAGGCCCTGGGGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 679 |
| LEAP-Seq percent confirming: | 99.8222 |
| LEAP-Seq n confirming: | 3369 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTCAGTACGCCATAGGTT |
| Suggested primer 2: | TTGTCTACTGGTGACGTGGC |