| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133695 |
| Chromosome: | chromosome 1 |
| Location: | 7897425 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g063632 | (1 of 1) K03660 - N-glycosylase/DNA lyase (OGG1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCGGGTCCGCCGCAGGGTGGGAAGTGC |
| Internal bar code: | CCAGTGGAATCTAGGATTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 99.4167 |
| LEAP-Seq n confirming: | 1193 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACGTCCTGAAAGTGCGT |
| Suggested primer 2: | TTGTTTTCAACACTCGCAGC |