| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133695 |
| Chromosome: | chromosome 2 |
| Location: | 7330531 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g145150 | MAM3A | (1 of 5) K16302 - metal transporter CNNM (CNNM); Protein putatively involved in mitochondrial biogenesis | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCGCACGGCAAAGCGGGGTAGCCGATC |
| Internal bar code: | GCGGTTCACCTGCTTTGGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 99.9605 |
| LEAP-Seq n confirming: | 5056 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCATTTCGTGATTTCGGG |
| Suggested primer 2: | GAGAAACAGAGCCAACAGGC |