| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133701 |
| Chromosome: | chromosome 6 |
| Location: | 152917 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g250200 | SAS1,METM,METM1 | (1 of 1) 2.5.1.6 - Methionine adenosyltransferase / S-adenosylmethionine synthetase; S-adenosylmethionine synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGGGTTGCCTAATGGTGCATGGGCCTT |
| Internal bar code: | GGCAAGGGAGGGACGTGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 86.2428 |
| LEAP-Seq n confirming: | 746 |
| LEAP-Seq n nonconfirming: | 119 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGCAGGTTGAGTGTCTA |
| Suggested primer 2: | GGTCTACAGCTGGGTGGGTA |