| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133717 |
| Chromosome: | chromosome 16 |
| Location: | 4219827 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668550 | (1 of 1) PF01363//PF04366//PF13920 - FYVE zinc finger (FYVE) // Las17-binding protein actin regulator (Ysc84) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCCTGTTTGTATTTGTGTCGGGGGGCA |
| Internal bar code: | CCAATCGGGGCGTCTGGCACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 310 |
| LEAP-Seq percent confirming: | 99.2451 |
| LEAP-Seq n confirming: | 2761 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGCTGGTATGCAGAAAT |
| Suggested primer 2: | ATCGGGTGACTGACTTGGTC |