| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133724 |
| Chromosome: | chromosome 6 |
| Location: | 1430369 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259150 | EFG8 | (1 of 1) PTHR23115//PTHR23115:SF155 - TRANSLATION FACTOR // SUBFAMILY NOT NAMED; Translation elongation factor Tu family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACCATGCAGCAAAATTCTGGTGCCTGTT |
| Internal bar code: | GCTTCATCATCTGAGCTTTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 99.5745 |
| LEAP-Seq n confirming: | 468 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGATGCTGGTGAAGTGAA |
| Suggested primer 2: | GTGTCGGGCTGAACGTTATT |