Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.133757 |
Chromosome: | chromosome_12 |
Location: | 2908683 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g501900 | FFT2 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTCAGGCCTTCTTGTCTGGGCCACGCTCCT |
Internal bar code: | TCGGAGACGACTGGCACTGCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 631 |
LEAP-Seq percent confirming: | 97.2364 |
LEAP-Seq n confirming: | 2287 |
LEAP-Seq n nonconfirming: | 65 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAGGTGGGTGTGAAGAAGG |
Suggested primer 2: | CCATGTAGTCCATCGCTGTG |