| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133852 |
| Chromosome: | chromosome 6 |
| Location: | 3042927 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274200 | HTA42,HTA6 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAATCTTTTCGCCTTTGTCACCCAGCCAG |
| Internal bar code: | CTGGCAAATCTCACGTCTACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 55 |
| LEAP-Seq percent confirming: | 59.5238 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAGAAGGGCAAGTACGC |
| Suggested primer 2: | GTCACCGATGTGAGTTGGTG |